What do we need when you send your samples to us?
- Trizol or Qiagen extracted total RNA are both fine for GeneChip analysis.
- Trizol extraction followed by a clean up step using the Qiagen columns usually removes most impurities.
- We strongly recommend you perform a clean-up protocol on all your samples before sending them to us.
- You can send your RNA in solution on dry ice or pelleted by ethanol precipitation and air dried.
- Resuspension of dried RNA can be variable. Therefore we suggest that you send RNA on dry ice. We will accept a minimum of 100 - 500ng total for each sample, concentrated to no less than 100ng in 3ul (we recommend 50ng/ul). If your preferred method is to air dry your samples we require approximately 1µg of dried total RNA for each GeneChip experiment where possible. You must let us know of the expected delivery time and date of samples transmitted on dry-ice.
- Please make sure your sample tubes are clearly labelled using the names used on the sample submission form.
- Our address is here
If you have any questions, please contact our Affymetrix team at affy@arabidopsis.info.
Small sample preparation.
We can also label small amounts of RNA by applying additional amplification. This requires between 10-100ng of total RNA.There is an additional charge of £50 per sample for small sample prep.
Please contact us if you would like to use this amplification service.
How much tissue is 100mg?
The estimates below are presented as a rough guide to the amount of tissue from an average Arabidopsis plant that constitutes 100mg of material.
Tissue was taken from an average of a small (3 plant) sample of moderately crowded Columbia plants between stages 6.00 - 6.10 [ Boyes et al, (2001)]. These were very slightly younger than the plant shown in the figure to the right with the first elongating and immature siliques only newly apparent.
These average measurements are rounded to the nearest 10mg.
-
Largest rosette leaf: ~40 mg each leaf
(3.5 to 4 cm length inc. petiole x 1 to 1.1 cm width) -
Cauline leaf: ~20 mg each leaf
(2 cm length x 1 cm width) -
Flowering tip: ~20 mg
(all buds, flowers, some fertilised with newly protruding styles)
(2 cm length x 1 cm width) -
Naked stem: ~130 mg
(including hypocotyl, but NO roots; leaves; flowers; nor lateral branches)