How much does it cost?
(£350* pounds per ATH1 or Human gene 1.0 ST or Human SNP 6.0 array including hyb)
We are happy to provide quotes for grants - arabidopsis, human or otherwise.
*UK and other European customers: please add your national sales tax where appropriate - e.g. VAT, TVA, MWST, MOMS [More information]. According to the Nottingham University Finance Department, this is a service and not 'goods' so medical users are NOT exempt from VAT.
- £350* for RNA onto any chip in GROUP A (RNA through to data).
- £450* for RNA onto any chip in GROUP B (RNA through to data).
- Special case: £350* for =DNA= onto any chip in group A OR B.
(e.g. for SNP genotyping, ChIP on chip, deletion, bulk-segregant analysis, ecotype, xspecies or other uses).
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GROUP A:
ATH1 (arabidopsis) Human gene 1.0 ST Human SNP 6.0 Bovine Tomato C. elegans Drosophila Anopheles Pig Sugar Grape Xenopus Barley Zebrafish
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GROUP B: Human plus 2.0 Exon chips Arabidopsis Tiling chips Wheat Citrus Dog Chicken Medicago |
Please ask for details of chips not listed.
If you supply your own chips then we will charge £150 all inclusive* per hybridisation.
If you have other requirements (e.g. DNA hybridisation for SNPs, deletion mapping, cross-species work; or you wish to supply your own labelled cRNA) then please apply for a quote to affy@arabidopsis.info if you would like to do this.
Small sample preparation. (add £50 per sample)
We can label small amounts of RNA using the Affymetrix two-cycle kit. This requires between 10-100ng of total RNA.This is NOT recommended for standard experiments.
+ What is included in the service?
- Pre-service advice and discussion
- Email acknowledgement receipt for RNA.
-
Total RNA Quality control (see the QC page
for more details).
- Nanodrop quantification check.
- Agilent bioanalyser analysis.
- QC report & email confirmation to proceed.
- cRNA Quality control & email confirmation to proceed if necessary.
- Affymetrix chip & labelling costs.
- Hybridisation & detection fluidics and materials.
- Scan, QC and data tracking.
- Data formatting (various)
- CD including Raw data and Excel formatted data.
- Discussion of data interpretation by email or visit.
- Six-month default confidentiality on plant data
When the confidentiality period has expired, we then...
- Free construction of XML/MAGE-ML and donation to ArrayExpress (EBI) (in your name as experimenter).
- Inclusion of your data into the NASCArrays database and integration into spot history, scatterplot and other on-line tools.
This is a valuable part of the service- most journals now demand release of data to a public database before microarray results can be published. This can be an arduous process. At NASC, we take care of this for you.
Non-plant experiments
If you perform non-plant experiments, then your data is confidential. The confidentiality period therefore does not apply. However please note that most funders now have a data release policy and we can submit your data on your behalf to repositories such as GEO and ArrayExpress. This is not a trivial process (as stated above).
How to apply
Use the application form to apply.
Disclaimer
We are not-for-profit and it is important that we make it clear that the service is treated as an academic collaboration without issues of warranty. We are a very professional unit and have a lot of experience now (especially in QC), however the service is NOT a commercial one and once we give you the data our formal responsibility is over (although we are normally happy to help informally with advice on analysis where it is useful to you). As the user of our service you are not paying for the data or service itself, you are providing cost-recovery on part of the full cost to us of the procedure. This helps the concept that the data ultimately belongs to the community since the community (and funding body) provide the underpinning finance and subsidy to NASC with this explicit understanding.