Please ask for details of chips not listed.
If you supply your own chips then we will charge £150 all inclusive* per
hybridisation.
If you have other requirements (e.g. DNA hybridisation for SNPs,
deletion mapping, cross-species work; or you wish to supply your own labelled
cRNA) then please apply for a quote to
affy@arabidopsis.info
if you would like to do this.
Small sample preparation. (add £50 per sample)
We can label small amounts of RNA using the Affymetrix two-cycle kit. This requires between 10-100ng of total RNA.
This is NOT recommended for standard experiments.
+ What is included in the service?
- Pre-service advice and discussion
- Email acknowledgement receipt for RNA.
-
Total RNA Quality control (see the QC page
for more details).
- Nanodrop quantification check.
- Agilent bioanalyser analysis.
- QC report & email confirmation to proceed.
- cRNA Quality control & email confirmation to proceed if necessary.
- Affymetrix chip & labelling costs.
- Hybridisation & detection fluidics and materials.
- Scan, QC and data tracking.
- Data formatting (various)
- CD including Raw data and Excel formatted data.
- Discussion of data interpretation by email or visit.
- Six-month default confidentiality on plant data
When the confidentiality period has expired, we then...
- Free construction of XML/MAGE-ML and donation to ArrayExpress
(EBI) (in your name as experimenter).
- Inclusion of your data into the NASCArrays database and integration into
spot history, scatterplot and other on-line tools.
This is a valuable part of the service- most journals now demand
release of data to a public database before microarray results can be
published. This can be an arduous process. At NASC, we take care of
this for you.
Non-plant experiments
If you perform non-plant experiments, then your data is confidential.
The confidentiality period therefore does not apply. However please note that
most funders now have a data release policy and we can submit your data on your
behalf to repositories such as GEO and ArrayExpress. This is not a trivial process
(as stated above).
How to apply
Use the application form to apply.
Disclaimer
We are not-for-profit and it is important that we make it clear that the
service is treated as an academic collaboration without issues of warranty.
We are a very professional unit and have a lot of experience now (especially in QC),
however the service is NOT a commercial one and once we give you the data our
formal responsibility is over (although we are normally happy to help
informally with advice on analysis where it is useful to you).
As the user of our service you are not paying for the data or service itself,
you are providing cost-recovery on part of the full cost to us of the
procedure. This helps the concept that the data ultimately belongs
to the community since the community (and funding body) provide the
underpinning finance and subsidy to NASC with this explicit understanding.
